Lisa MARTINEZ
Doctorante Université
novembre 2019 - mars 2023
| Équipe : |
Thèmes de recherche
Titre de la thèse « Etude des sols dans l’interaction entre l’orobanche rameuse et le colza d’hiver »
Etude de la diversité microbienne des sols et des signaux allélopathiques rhizosphériques en lien avec le parasitisme de l’orobanche.
Projets
Parcours universitaire
2013-2016 : Classe préparatoire BCPST, lycée François Premier (77, Fontainebleau)
2016-2019 : Ecole d’ingénieur en agronomie Agrocampus Ouest (35, Rennes)
Publications
2 publications
Bendejacq-Seychelles, Ana; Martinez, Lisa; Corréard, Anaïs; Totozafy, Jean Chrisologue; Steinberg, Christian; Pouvreau, Jean-Bernard; Reibel, Carole; Mouille, Grégory; Mondy, Samuel; Poulin, Lucie; Gibot-Leclerc, Stéphanie
Image Analysis and Untargeted Metabolomics Reveal Potential Phytotoxins from Against Major Parasitic Weed (L.) Pomel Article de journal
Dans: Toxins (Basel), vol. 16, no. 12, 2024, ISSN: 2072-6651.
@article{pmid39728789,
title = {Image Analysis and Untargeted Metabolomics Reveal Potential Phytotoxins from Against Major Parasitic Weed (L.) Pomel},
author = {Ana Bendejacq-Seychelles and Lisa Martinez and Anaïs Corréard and Jean Chrisologue Totozafy and Christian Steinberg and Jean-Bernard Pouvreau and Carole Reibel and Grégory Mouille and Samuel Mondy and Lucie Poulin and Stéphanie Gibot-Leclerc},
doi = {10.3390/toxins16120531},
issn = {2072-6651},
year = {2024},
date = {2024-12-01},
urldate = {2024-12-01},
journal = {Toxins (Basel)},
volume = {16},
number = {12},
abstract = {Branched broomrape ( (L.) Pomel), an obligate parasitic weed with a wide host range, is known for its devasting effects on many crops worldwide. Soil fungi, notably sp., are described as pathogenic to broomrape, while the hypothesis of the phytotoxicity of fusaric acid produced by for parasitic weeds of the genus has been proposed. Using image analysis and untargeted metabolomics, this study investigated fungal metabolites phytotoxic for and produced by the MIAE02836 strain, isolated from symptomatic broomrapes and identified as a promising candidate for broomrape biocontrol. Phytotoxicity tests of crude extracts from the fungus alone or in interaction with broomrape on microcalli and quantification of necrosis by image analysis confirmed the phytotoxic potential of MIAE02836 metabolites towards the early developmental stages of . Data analysis of a non-targeted metabolomics approach revealed numerous metabolites produced by MIAE02836. Four of them, accumulated during interaction with the parasitic plant, are known for their phytotoxic potential: maculosin, cyclo(Leu-Phe), phenylalanyl-D-histidine and anguidine. These results suggest that combining image acquisition of the microcalli screening test and untargeted metabolomic approach is an interesting and relevant method to characterize phytotoxic fungal metabolites.},
keywords = {},
pubstate = {published},
tppubtype = {article}
}
Branched broomrape ( (L.) Pomel), an obligate parasitic weed with a wide host range, is known for its devasting effects on many crops worldwide. Soil fungi, notably sp., are described as pathogenic to broomrape, while the hypothesis of the phytotoxicity of fusaric acid produced by for parasitic weeds of the genus has been proposed. Using image analysis and untargeted metabolomics, this study investigated fungal metabolites phytotoxic for and produced by the MIAE02836 strain, isolated from symptomatic broomrapes and identified as a promising candidate for broomrape biocontrol. Phytotoxicity tests of crude extracts from the fungus alone or in interaction with broomrape on microcalli and quantification of necrosis by image analysis confirmed the phytotoxic potential of MIAE02836 metabolites towards the early developmental stages of . Data analysis of a non-targeted metabolomics approach revealed numerous metabolites produced by MIAE02836. Four of them, accumulated during interaction with the parasitic plant, are known for their phytotoxic potential: maculosin, cyclo(Leu-Phe), phenylalanyl-D-histidine and anguidine. These results suggest that combining image acquisition of the microcalli screening test and untargeted metabolomic approach is an interesting and relevant method to characterize phytotoxic fungal metabolites.
Martinez, Lisa; Pouvreau, Jean-Bernard; Jestin, Christophe; Montiel, Gregory; Gravot, Antoine; Berardocco, Solenne; Marnet, Nathalie; Bouchereau, Alain; Delage, Erwan; Simier, Philippe; Poulin, Lucie
Comparative Analysis of Two Neighboring Conducive and Suppressive Soils Toward Plant Parasitism Caused by Phelipanche ramosa on Brassica napus Article de journal
Dans: Phytobiomes Journal, vol. 8, no. 4, p. 425–445, 2024, ISSN: 2471-2906.
@article{Martinez2024,
title = {Comparative Analysis of Two Neighboring Conducive and Suppressive Soils Toward Plant Parasitism Caused by \textit{Phelipanche ramosa} on \textit{Brassica napus}},
author = {Lisa Martinez and Jean-Bernard Pouvreau and Christophe Jestin and Gregory Montiel and Antoine Gravot and Solenne Berardocco and Nathalie Marnet and Alain Bouchereau and Erwan Delage and Philippe Simier and Lucie Poulin},
doi = {10.1094/pbiomes-12-23-0140-r},
issn = {2471-2906},
year = {2024},
date = {2024-11-00},
urldate = {2024-11-00},
journal = {Phytobiomes Journal},
volume = {8},
number = {4},
pages = {425--445},
publisher = {Scientific Societies},
abstract = {<jats:p> In Western France, rapeseed ( Brassica napus) cultivation faces substantial yield losses due to the root holoparasitic plant Phelipanche ramosa. However, recent observations have shown a reduction in parasitism within previously heavily infested fields. This study investigates two neighboring rapeseed soils with distinct levels of parasitic infestation, considered suppressive and conducive. Using a cocultivation system of Brassica napus and P. ramosa, we comprehensively examined rhizosphere exudates, parasitic plant attachment, and rhizosphere soil microbiota. Our findings revealed that the suppressive soil effectively reduced parasitism by impeding broomrape attachment and development, as well as inducing necrosis of tubercles. This suppressive effect was specific to postattachment stages, leaving germination and haustoriogenesis preattachment stages unaffected. Analysis of microbial structures suggested that the suppression of parasitism is predominantly of fungal rather than bacterial origin. Correlation network analyses identified three groups of amplicon sequence variants (ASVs) associated with suppression. Notably, seven ASVs were inversely correlated with parasitic attachments, and only one ASV, identified as Berkeleyomyces, a necrotrophic fungus responsible for black root rot, was positively correlated with necrosis and was more abundant in the suppressive soil. This study demonstrates the contrasting parasitic plant development on two physicochemically similar soils, highlighting the central role of fungal dynamics in the rhizosphere. These results provide valuable insights into the mechanisms underlying soil-mediated suppression of P. ramosa, offering potential strategies for mitigating the impact of this root holoparasite on rapeseed yields in the region. </jats:p>},
keywords = {},
pubstate = {published},
tppubtype = {article}
}
<jats:p> In Western France, rapeseed ( Brassica napus) cultivation faces substantial yield losses due to the root holoparasitic plant Phelipanche ramosa. However, recent observations have shown a reduction in parasitism within previously heavily infested fields. This study investigates two neighboring rapeseed soils with distinct levels of parasitic infestation, considered suppressive and conducive. Using a cocultivation system of Brassica napus and P. ramosa, we comprehensively examined rhizosphere exudates, parasitic plant attachment, and rhizosphere soil microbiota. Our findings revealed that the suppressive soil effectively reduced parasitism by impeding broomrape attachment and development, as well as inducing necrosis of tubercles. This suppressive effect was specific to postattachment stages, leaving germination and haustoriogenesis preattachment stages unaffected. Analysis of microbial structures suggested that the suppression of parasitism is predominantly of fungal rather than bacterial origin. Correlation network analyses identified three groups of amplicon sequence variants (ASVs) associated with suppression. Notably, seven ASVs were inversely correlated with parasitic attachments, and only one ASV, identified as Berkeleyomyces, a necrotrophic fungus responsible for black root rot, was positively correlated with necrosis and was more abundant in the suppressive soil. This study demonstrates the contrasting parasitic plant development on two physicochemically similar soils, highlighting the central role of fungal dynamics in the rhizosphere. These results provide valuable insights into the mechanisms underlying soil-mediated suppression of P. ramosa, offering potential strategies for mitigating the impact of this root holoparasite on rapeseed yields in the region. </jats:p>
1 publication
Martinez, Lisa; Pouvreau, Jean-Bernard; Montiel, Gregory; Jestin, Christophe; Delavault, Philippe; Simier, Philippe; Poulin, Lucie
Soil microbiota promotes early developmental stages of Phelipanche ramosa L. Pomel during plant parasitism on Brassica napus L. Article de journal
Dans: Plant and Soil, vol. 483, p. 667–691 , 2022.
@article{martinez2022soil,
title = {Soil microbiota promotes early developmental stages of Phelipanche ramosa L. Pomel during plant parasitism on Brassica napus L.},
author = {Lisa Martinez and Jean-Bernard Pouvreau and Gregory Montiel and Christophe Jestin and Philippe Delavault and Philippe Simier and Lucie Poulin},
url = {https://nantes-universite.hal.science/hal-04370677v1 },
doi = {https://doi.org/10.1007/s11104-022-05822-6},
year = {2022},
date = {2022-12-08},
urldate = {2022-12-08},
journal = {Plant and Soil},
volume = {483},
pages = {667–691 },
publisher = {Springer},
abstract = {Purpose
The root holoparasitic plant Phelipanche ramosa has become a major constraint for rapeseed cultivation in western France for the last decades and its control remains challenging. To date, few studies have considered soil microbiota as a third partner of the parasitic plant-plant interaction. Therefore, we here addressed the question of how soil microbiota interferes with host-derived signal metabolites required for host plant recognition by the parasitic plant.
Methods
Using a branched broomrape infested soil (genetic group 1) from a rapeseed field, we first provided soil physicochemical and microbiological descriptions by metabarcoding, followed by P. ramosa seed germination and prehaustorium formation bioassays, and by in vitro co-cultivation with Brassica napus.
Results
Co-cultivation in presence of soil microorganisms promoted parasitic plant seed germination and attachments to host’s roots. Seed germination assays showed that only the combination of gluconasturtiin (main rapeseed glucosinolate) with soil extracts stimulated broomrape germination. This suggests a microbial conversion of gluconasturtiin into germination stimulants via soil microbial myrosinase enzymes. Furthermore, soil bacteria Arthrobacter, Ralstonia, Actinobacterium, Proteobacterium spp. and fungus Penicillium spp. were isolated and screened for myrosinase activity. Pre-germinated seeds treated with soil extracts or differentially filtrated soil extracts also promoted the formation of P. ramosa prehaustorium and led to more parasitic attachments on rapeseed roots in co-cultivation assays. This thus suggests that this enhancement of parasitic attachments could also be partly attributed to soil microbial production of haustorium inducing factors.
Conclusion
Soil microbiota influences B. napus - P. ramosa interaction by altering direct and indirect recognition signals.},
keywords = {},
pubstate = {published},
tppubtype = {article}
}
Purpose
The root holoparasitic plant Phelipanche ramosa has become a major constraint for rapeseed cultivation in western France for the last decades and its control remains challenging. To date, few studies have considered soil microbiota as a third partner of the parasitic plant-plant interaction. Therefore, we here addressed the question of how soil microbiota interferes with host-derived signal metabolites required for host plant recognition by the parasitic plant.
Methods
Using a branched broomrape infested soil (genetic group 1) from a rapeseed field, we first provided soil physicochemical and microbiological descriptions by metabarcoding, followed by P. ramosa seed germination and prehaustorium formation bioassays, and by in vitro co-cultivation with Brassica napus.
Results
Co-cultivation in presence of soil microorganisms promoted parasitic plant seed germination and attachments to host’s roots. Seed germination assays showed that only the combination of gluconasturtiin (main rapeseed glucosinolate) with soil extracts stimulated broomrape germination. This suggests a microbial conversion of gluconasturtiin into germination stimulants via soil microbial myrosinase enzymes. Furthermore, soil bacteria Arthrobacter, Ralstonia, Actinobacterium, Proteobacterium spp. and fungus Penicillium spp. were isolated and screened for myrosinase activity. Pre-germinated seeds treated with soil extracts or differentially filtrated soil extracts also promoted the formation of P. ramosa prehaustorium and led to more parasitic attachments on rapeseed roots in co-cultivation assays. This thus suggests that this enhancement of parasitic attachments could also be partly attributed to soil microbial production of haustorium inducing factors.
Conclusion
Soil microbiota influences B. napus - P. ramosa interaction by altering direct and indirect recognition signals.
The root holoparasitic plant Phelipanche ramosa has become a major constraint for rapeseed cultivation in western France for the last decades and its control remains challenging. To date, few studies have considered soil microbiota as a third partner of the parasitic plant-plant interaction. Therefore, we here addressed the question of how soil microbiota interferes with host-derived signal metabolites required for host plant recognition by the parasitic plant.
Methods
Using a branched broomrape infested soil (genetic group 1) from a rapeseed field, we first provided soil physicochemical and microbiological descriptions by metabarcoding, followed by P. ramosa seed germination and prehaustorium formation bioassays, and by in vitro co-cultivation with Brassica napus.
Results
Co-cultivation in presence of soil microorganisms promoted parasitic plant seed germination and attachments to host’s roots. Seed germination assays showed that only the combination of gluconasturtiin (main rapeseed glucosinolate) with soil extracts stimulated broomrape germination. This suggests a microbial conversion of gluconasturtiin into germination stimulants via soil microbial myrosinase enzymes. Furthermore, soil bacteria Arthrobacter, Ralstonia, Actinobacterium, Proteobacterium spp. and fungus Penicillium spp. were isolated and screened for myrosinase activity. Pre-germinated seeds treated with soil extracts or differentially filtrated soil extracts also promoted the formation of P. ramosa prehaustorium and led to more parasitic attachments on rapeseed roots in co-cultivation assays. This thus suggests that this enhancement of parasitic attachments could also be partly attributed to soil microbial production of haustorium inducing factors.
Conclusion
Soil microbiota influences B. napus - P. ramosa interaction by altering direct and indirect recognition signals.